Acceptor framework for cdr grafting

ABSTRACT

The present invention relates to an antibody acceptor framework and to methods for grafting non-human antibodies, e.g., rabbit antibodies, using a particularly well suited antibody acceptor framework. Antibodies generated by the methods of the invention are useful in a variety of diagnostic and therapeutic applications.

The present application is a divisional application of U.S. applicationSer. No. 12/823,488 filed Jun. 25, 2010 (now allowed), which claims thebenefit of U.S. Provisional Patent Application Ser. No. 61/220,503,filed on Jun. 25, 2009, the disclosure of which is specificallyincorporated by reference herein.

BACKGROUND OF THE INVENTION

Monoclonal antibodies, their conjugates and derivatives are hugelycommercially important as therapeutic and diagnostic agents. Non-humanantibodies elicit a strong immune response in patients, usuallyfollowing a single low dose injection (Schroff, 1985 Cancer Res45:879-85, Shawler. J Immunol 1985 135:1530-5; Dillman, Cancer Biother1994 9:17-28). Accordingly, several methods for reducing theimmunogenicity of murine and other rodent antibodies as well astechnologies to make fully human antibodies using e.g. transgenic miceor phage display were developed. Chimeric antibodies were engineered,which combine rodent variable regions with human constant regions (e.g.,Boulianne Nature 1984 312:643-6) reduced immunogenicity problemsconsiderably (e.g., LoBuglio, Proc Natl Acad Sci 1989 86:4220-4; Clark,Immunol Today 2000 21:397-402). Humanized antibodies were alsoengineered, in which the rodent sequence of the variable region itselfis engineered to be as close to a human sequence as possible whilepreserving at least the original CDRs, or where the CDRs from the rodentantibody were grafted into framework of a human antibody (e.g.,Riechmann, Nature 1988 332:323-7; U.S. Pat. No. 5,693,761). Rabbitpolyclonal antibodies are widely used for biological assays such asELISAs or Western blots. Polyclonal rabbit antibodies are oftentimesfavored over polyclonal rodent antibodies because of their usually muchhigher affinity. Furthermore, rabbit oftentimes are able to elicit goodantibody responses to antigens that are poorly immunogenic in miceand/or which give not rise to good binders when used in phage display.Due to these well-known advantages of rabbit antibodies, they would beideal to be used in the discovery and development of therapeuticantibodies. The reason that this is not commonly done is mainly due totechnical challenges in the generation of monoclonal rabbit antibodies.Since myeloma-like tumors are unknown in rabbits, the conventionalhybridoma technology to generate monoclonal antibodies is not applicableto rabbit antibodies. Pioneering work in providing fusion cell linepartners for rabbit antibody-expressing cells has been done by Knightand colleagues (Spieker-Polet et al., PNAS 1995, 92:9348-52) and animproved fusion partner cell line has been described by Pytela et al. in2005 (see e.g. U.S. Pat. No. 7,429,487). This technology, however, isnot widely spread since the corresponding know-how is basicallycontrolled by a single research group. Alternative methods for thegeneration of monoclonal antibodies that involve the cloning ofantibodies from selected antibody-expressing cells via RT-PCR aredescribed in the literature, but have never been successfully reportedfor rabbit antibodies.

Rabbit antibodies, like mouse antibodies are expected to elicit strongimmune responses if used for human therapy, thus, rabbit antibodies needto be humanized before they can be used clinically. However, the methodsthat are used to make humanized rodent antibodies cannot easily beextrapolated for rabbit antibodies due to structural differences betweenrabbit and mouse and, respectively, between rabbit and human antibodies.For example, the light chain CDR3 (CDRL3) is often much longer thanpreviously known CDRL3s from human or mouse antibodies.

There are few rabbit antibody humanization approaches described in theprior art, which are, however, no classical grafting approach in whichthe CDRs of a non-human donor are transplanted on a human acceptorantibody. WO 04/016740 describes a so-called “resurfacing” strategy. Thegoal of a “resurfacing” strategy is to remodel the solvent-accessibleresidues of the non-human framework such that they become morehuman-like. Similar humanization techniques for rabbit antibodies asdescribed in WO 04/016740 are known in the art. Both WO08/144757 andWO05/016950 disclose methods for humanizing a rabbit monoclonal antibodywhich involve the comparison of amino acid sequences of a parent rabbitantibody to the amino acid sequences of a similar human antibody.Subsequently, the amino acid sequence of the parent rabbit antibody isaltered such that its framework regions are more similar in sequence tothe equivalent framework regions of the similar human antibody. In orderto gain good binding capacities, laborious development efforts need tobe made for each immunobinder individually.

A potential problem of the above-described approaches is that not ahuman framework is used, but the rabbit framework is engineered suchthat it looks more human-like. Such approach carries the risk that aminoacid stretches that are buried in the core of the protein still mightcomprise immunogenic T cell epitopes.

To date, the applicants have not identified a rabbit antibody, which washumanized by applying state-of-the-art grafting approaches. This mightbe explained by fact that rabbit CDRs may be quite different from humanor rodent CDRs. As known in the art, many rabbit VH chains have extrapaired cysteines relative to the murine and human counterparts. Inaddition to the conserved disulfide bridge formed between cys22 andcys92, there is also a cys21-cys79 bridge as well as an interCDR S-Sbridge formed between the last residue of CDRH1 and the first residue ofCDR H2 in some rabbit chains. Besides, pairs of cysteine residues areoften found in the CDR-L3. Moreover, many rabbit antibody CDRs do notbelong to any previously known canonical structure. In particular theCDR-L3 is often much longer than the CDR-L3 of a human or murinecounterpart.

Hence, the grafting of non-human CDRs antibodies into a human frameworkis a major protein engineering task. The transfer of antigen bindingloops from a naturally evolved framework to a different artificiallyselected human framework must be performed so that native loopconformations are retained for antigen binding. Often antigen bindingaffinity is greatly reduced or abolished after loop grafting. The use ofcarefully selected human frameworks in grafting the antigen bindingloops maximizes the probability of retaining binding affinity in thehumanized molecule (Roguzka et al 1996). Although the many graftingexperiments available in the literature provide a rough guide for CDRgrafting, it is not possible to generalize a pattern. Typical problemsconsist in loosing the specificity, stability or producibility aftergrafting the CDR loops.

Accordingly, there is an urgent need for improved methods for reliablyand rapidly humanizing rabbit antibodies for use as therapeutic anddiagnostic agents. Furthermore, there is a need for human acceptorframeworks for reliably humanizing rabbit antibodies, providingfunctional antibodies and/or antibody fragments with drug-likebiophysical properties.

SUMMARY OF THE INVENTION

It has surprisingly been found that a highly soluble and stable humanantibody framework identified by a Quality Control (QC) assay (asdisclosed in WO 0148017 and in Auf der Maur et al (2001), FEBS Lett 508,p. 407-412) is particularly suitable for accommodating CDRs from othernon-human animal species, for example, rabbit CDRs. Accordingly, in afirst aspect, the invention provides the heavy chain variable regions ofa particular human antibody (the so called, “a72” VH framework sequence)which is especially suitable as acceptor for CDRs from a variety ofantibodies, in particular from rabbit antibodies, of different bindingspecificities, independent of whether a disulfide bridge is present in aCDR or not.

Humanized immunobinders generated by the grafting of rabbit CDRs intothis highly compatible variable chain framework consistently andreliably retain the spatial orientation of the rabbit antibodies fromwhich the donor CDRs are derived. Therefore, no structurally relevantpositions of the donor immunobinder need to be introduced into theacceptor framework. Due to these advantages, high-throughputhumanization of rabbit antibodies with no or little optimization of thebinding capacities can be achieved.

Accordingly, in another aspect, the invention provides methods forgrafting rabbit and other non-human CDRs, into the soluble and stablelight chain and/or heavy chain human antibody framework sequencesdisclosed herein, thereby generating humanized antibodies with superiorbiophysical properties. In particular, immunobinders generated by themethods of the invention exhibit superior functional properties such assolubility and stability.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts the CDR H1 definition used herein for grafting antigenbinding sites from rabbit monoclonal antibodies into the highly solubleand stable human antibody frameworks.

FIG. 2: An analysis of rabbit antibody sequences extracted from theKabat database confirms that CDR3 of the variable heavy chain istypically by three amino acids longer than its murine counterpart.

DETAILED DESCRIPTION OF THE INVENTION Definitions

In order that the present invention may be more readily understood,certain terms will be defined as follows. Additional definitions are setforth throughout the

DETAILED DESCRIPTION

The term “antibody” refers to whole antibodies and any antigen bindingfragment. The term “antigen binding polypeptide” and “immunobinder” areused simultaneously herein. An “antibody” refers to a protein,optionally glycosylated, comprising at least two heavy (H) chains andtwo light (L) chains inter-connected by disulfide bonds, or an antigenbinding portion thereof. Each heavy chain is comprised of a heavy chainvariable region (abbreviated herein as V_(H)) and a heavy chain constantregion. The heavy chain constant region is comprised of three domains,CH1, CH2 and CH3. Each light chain is comprised of a light chainvariable region (abbreviated herein as V_(L)) and a light chain constantregion. The light chain constant region is comprised of one domain, CL.The V_(H) and V_(L) regions can be further subdivided into regions ofhypervariability, termed complementarity determining regions (CDR),interspersed with regions that are more conserved, termed frameworkregions (FR). Each V_(H) and V_(L) is composed of three CDRs and fourFRs, arranged from amino-terminus to carboxy-terminus in the followingorder: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The variable regions of theheavy and light chains contain a binding domain that interacts with anantigen. The constant regions of the antibodies may mediate the bindingof the immunoglobulin to host tissues or factors, including variouscells of the immune system (e.g., effector cells) and the firstcomponent (Clq) of the classical complement system.

The term “antigen-binding portion” of an antibody (or simply “antibodyportion”) refers to one or more fragments of an antibody that retain theability to specifically bind to an antigen (e.g., TNF). It has beenshown that the antigen-binding function of an antibody can be performedby fragments of a full-length antibody.

Examples of binding fragments encompassed within the term“antigen-binding portion” of an antibody include (i) a Fab fragment, amonovalent fragment consisting of the V_(L), V_(H), CL and CH1 domains;(ii) a F(ab′)₂ fragment, a bivalent fragment comprising two Fabfragments linked by a disulfide bridge at the hinge region; (iii) a Fdfragment consisting of the V_(H) and CH1 domains; (iv) a Fv fragmentconsisting of the V_(L) and V_(H) domains of a single arm of anantibody, (v) a single domain or dAb fragment (Ward et al., (1989)Nature 341:544-546), which consists of a V_(H) domain; and (vi) anisolated complementarity determining region (CDR) or (vii) a combinationof two or more isolated CDRs which may optionally be joined by asynthetic linker. Furthermore, although the two domains of the Fvfragment, V_(L) and V_(H), are coded for by separate genes, they can bejoined, using recombinant methods, by a synthetic linker that enablesthem to be made as a single protein chain in which the V_(L) and V_(H)regions pair to form monovalent molecules (known as single chain Fv(scFv); see e.g., Bird et al. (1988) Science 242:423-426; and Huston etal. (1988) Proc. Natl. Acad. Sci. USA 85:5879-5883). Such single chainantibodies are also intended to be encompassed within the term“antigen-binding portion” of an antibody. These antibody fragments areobtained using conventional techniques known to those with skill in theart, and the fragments are screened for utility in the same manner asare intact antibodies. Antigen-binding portions can be produced byrecombinant DNA techniques, or by enzymatic or chemical cleavage ofintact immunoglobulins. Antibodies can be of different isotype, forexample, an IgG (e.g., an IgG1, IgG2, IgG3, or IgG4 subtype), IgA1,IgA2, IgD, IgE, or IgM antibody.

The term “immunobinder” refers to a molecule that contains all or a partof the antigen binding site of an antibody, e.g. all or part of theheavy and/or light chain variable domain, such that the immunobinderspecifically recognizes a target antigen. Non-limiting examples ofimmunobinders include full-length immunoglobulin molecules and scFvs, aswell as antibody fragments, including but not limited to (i) a Fabfragment, a monovalent fragment consisting of the V_(L), V_(H), C_(L)and C_(H)1 domains; (ii) a F(ab′)₂ fragment, a bivalent fragmentcomprising two Fab fragments linked by a disulfide bridge at the hingeregion; (iii) a Fab′ fragment, which is essentially a Fab with part ofthe hinge region (see, FUNDAMENTAL IMMUNOLOGY (Paul ed., 3.sup.rd ed.1993); (iv) a Fd fragment consisting of the V_(H) and C_(H)1 domains;(v) a Fv fragment consisting of the V_(L) and V_(H) domains of a singlearm of an antibody, (vi) a single domain antibody such as a Dab fragment(Ward et al., (1989) Nature 341:544-546), which consists of a V_(H) orV_(L) domain, a Camelid (see Hamers-Casterman, et al., Nature363:446-448 (1993), and Dumoulin, et al., Protein Science 11:500-515(2002)) or a Shark antibody (e.g., shark Ig-NARs Nanobodies®; and (vii)a nanobody, a heavy chain variable region containing a single variabledomain and two constant domains.

The term “single chain antibody”, “single chain Fv” or “scFv” refers toa molecule comprising an antibody heavy chain variable domain (orregion; V_(H)) and an antibody light chain variable domain (or region;V_(L)) connected by a linker. Such scFv molecules can have the generalstructures: NH₂—V_(L)-linker-V_(H)—COOH or NH₂—V_(H)-linker-V_(L)—COOH.A suitable state of the art linker consists of repeated GGGGS amino acidsequences or variants thereof. In a preferred embodiment of the presentinvention a (GGGGS)₄ linker of the amino acid sequence set forth in SEQID NO: 8 is used, but variants of 1-3 repeats are also possible(Holliger et al. (1993), Proc. Natl. Acad. Sci. USA 90:6444-6448). Otherlinkers that can be used for the present invention are described byAlfthan et al. (1995), Protein Eng. 8:725-731, Choi et al. (2001), Eur.J. Immunol. 31:94-106, Hu et al. (1996), Cancer Res. 56:3055-3061,Kipriyanov et al. (1999), J. Mol. Biol. 293:41-56 and Roovers et al.(2001), Cancer Immunol.

As used herein, the term “functional property” is a property of apolypeptide (e.g., an immunobinder) for which an improvement (e.g.,relative to a conventional polypeptide) is desirable and/or advantageousto one of skill in the art, e.g., in order to improve the manufacturingproperties or therapeutic efficacy of the polypeptide. In oneembodiment, the functional property is stability (e.g., thermalstability). In another embodiment, the functional property is solubility(e.g., under cellular conditions). In yet another embodiment, thefunctional property is aggregation behavior. In still anotherembodiment, the functional property is protein expression (e.g., in aprokaryotic cell). In yet another embodiment the functional property isrefolding behavior following inclusion body solubilization in amanufacturing process. In certain embodiments, the functional propertyis not an improvement in antigen binding affinity. In another preferredembodiment, the improvement of one or more functional properties has nosubstantial effect on the binding affinity of the immunobinder.

The term “CDR” refers to one of the six hypervariable regions within thevariable domains of an antibody that mainly contribute to antigenbinding. One of the most commonly used definitions for the six CDRs wasprovided by Kabat E. A. et al., (1991) Sequences of proteins ofimmunological interest. NIH Publication 91-3242). As used herein,Kabat's definition of CDRs only apply for CDR1, CDR2 and CDR3 of thelight chain variable domain (CDR L1, CDR L2, CDR L3, or L1, L2, L3), aswell as for CDR2 and CDR3 of the heavy chain variable domain (CDR H2,CDR H3, or H2, H3). CDR1 of the heavy chain variable domain (CDR H1 orH1), however, as used herein is defined by the residue positions (Kabatnumbering) starting with position 26 and ending prior to position 36.This definition is basically a fusion of CDR H1 as differently definedby Kabat and Chotia (see also FIG. 1 for illustration).

The term “antibody framework” as used herein refers to the part of thevariable domain, either VL or VH, which serves as a scaffold for theantigen binding loops (CDRs) of this variable domain. In essence it isthe variable domain without the CDRs.

The term “epitope” or “antigenic determinant” refers to a site on anantigen to which an immunoglobulin or antibody specifically binds (e.g.,a specific site on the TNF molecule). An epitope typically includes atleast 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 consecutive ornon-consecutive amino acids in a unique spatial conformation. See, e.g.,Epitope Mapping Protocols in Methods in Molecular Biology, Vol. 66, G.E. Morris, Ed. (1996).

The terms “specific binding,” “selective binding,” “selectively binds,”and “specifically binds,” refer to antibody binding to an epitope on apredetermined antigen. Typically, the antibody binds with an affinity(K_(D)) of approximately less than 10⁻⁷ M, such as approximately lessthan 10 ⁻⁸ M, 10⁻⁹ M or 10⁻¹⁰ M or even lower.

The term “K_(D)” or “Kd” refers to the dissociation equilibrium constantof a particular antibody-antigen interaction. Typically, the antibodiesof the invention bind to TNF with a dissociation equilibrium constant(K_(D)) of less than approximately 10⁻⁷ M, such as less thanapproximately 10⁻⁸ M, 10⁻⁹ M or 10⁻¹⁰ M or even lower, for example, asdetermined using surface plasmon resonance (SPR) technology in a BIACOREinstrument.

The term “nucleic acid molecule,” as used herein refers to DNA moleculesand RNA molecules. A nucleic acid molecule may be single-stranded ordouble-stranded, but preferably is double-stranded DNA. A nucleic acidis “operably linked” when it is placed into a functional relationshipwith another nucleic acid sequence. For instance, a promoter or enhanceris operably linked to a coding sequence if it affects the transcriptionof the sequence.

The term “vector,” refers to a nucleic acid molecule capable oftransporting another nucleic acid to which it has been linked. In oneembodiment, the vector is a “plasmid,” which refers to a circular doublestranded DNA loop into which additional DNA segments may be ligated. Inanother embodiment, the vector is a viral vector, wherein additional DNAsegments may be ligated into the viral genome. The vectors disclosedherein can be capable of autonomous replication in a host cell intowhich they are introduced (e.g., bacterial vectors having a bacterialorigin of replication and episomal mammalian vectors) or can be can beintegrated into the genome of a host cell upon introduction into thehost cell, and thereby are replicated along with the host genome (e.g.,non-episomal mammalian vectors).

The term “host cell” refers to a cell into which an expression vectorhas been introduced. Host cells include bacterial, microbial, plant oranimal cells, preferably, Escherichia coli, Bacillus subtilis;Saccharomyces cerevisiae, Pichia pastoris, CHO (Chinese Hamster Ovarylines) or NSO cells.

The term “lagomorphs” refers to members of the taxonomic orderLagomorpha, comprising the families Leporidae (e.g. hares and rabbits),and the Ochotonidae (pikas). In a most preferred embodiment, thelagomorphs is a rabbit. The term “rabbit” as used herein refers to ananimal belonging to the family of the leporidae.

As used herein, “identity” refers to the sequence matching between twopolypeptides, molecules or between two nucleic acids. When a position inboth of the two compared sequences is occupied by the same base or aminoacid monomer subunit (for instance, if a position in each of twopolypeptides is occupied by a lysine), then the respective molecules areidentical at that position. The “percentage identity” between twosequences is a function of the number of identical positions shared bythe sequences, taking into account the number of gaps, and the length ofeach gap, which need to be introduced for optimal alignment of the twosequences. Generally, a comparison is made when two sequences arealigned to give maximum identity. Such alignment can be provided using,for instance, the method of the Needleman and Wunsch (J. Mol. Biol.(48):444-453 (1970)) algorithm which has been incorporated into the GAPprogram in the GCG software package, using either a Blossum 62 matrix ora PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and alength weight of 1, 2, 3, 4, 5, or 6.

Unless otherwise defined, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which this invention belongs. Although methods and materialssimilar or equivalent to those described herein can be used in thepractice or testing of the present invention, suitable methods andmaterials are described below. In case of conflict, the presentspecification, including definitions, will control. In addition, thematerials, methods, and examples are illustrative only and not intendedto be limiting.

Various aspects of the invention are described in further detail in thefollowing subsections. It is understood that the various embodiments,preferences and ranges may be combined at will. Further, depending ofthe specific embodiment, selected definitions, embodiments or ranges maynot apply.

If not otherwise stated, the amino acid positions are indicatedaccording to the AHo numbering scheme. The AHo numbering system isdescribed further in Honegger, A. and Pluckthun, A. (2001) J. Mol. Biol.309:657-670). Alternatively, the Kabat numbering system as describedfurther in Kabat et al. (Kabat, E. A., et al. (1991) Sequences ofProteins of Immunological Interest, Fifth Edition, U.S. Department ofHealth and Human Services, NIH Publication No. 91-3242) may be used.Conversion tables for the two different numbering systems used toidentify amino acid residue positions in antibody heavy and light chainvariable regions are provided in A. Honegger, J. Mol. Biol. 309 (2001)657-670.

In a first aspect, the present invention provides a human acceptorframework sequence for the grafting of CDRs from lagomorph species, forexample, from rabbit. The human single-chain VH framework a72 (SEQ IDNO: 1) was surprisingly found to be in essence highly compatible withthe antigen-binding sites of rabbit antibodies. Therefore, the a72 VHrepresents a suitable scaffold to construct stable humanized scFvantibody fragments derived from grafting of rabbit loops.

Thus, in one aspect, the invention provides an immunobinder acceptorframework, comprising a VH sequence having at least 70% identity to SEQID No. 1.

Said sequence may be combined with any other suitable variable lightchain. A preferred variable light chain is SEQ ID NO: 2 which was alsodisclosed in WO03/097697 and designated KI27, or any other VL sequenceas disclosed in WO03/097697.

In a preferred embodiment, the variable heavy chain framework is linkedto a variable light chain framework via a linker. The linker may be anysuitable linker, for example a linker comprising 1 to 4 repeats of thesequence GGGGS (SEQ ID NO: 5), preferably a (GGGGS)₄ peptide (SEQ ID NO:4), or a linker as disclosed in Alfthan et al. (1995) Protein Eng.8:725-731.

Accordingly, the present invention provides an immunobinder acceptorframework comprising

(i) a variable heavy chain framework having at least 70% identity,preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%identity, to SEQ ID NO: 1; and/or

(ii) a variable light chain framework having at least 70% identity,preferably at least 75%, 80%, 85%, 90%, more preferably at least 95%identity, to SEQ ID NO: 2.

In a much preferred embodiment, the invention provides an immunobinder,having a sequence with at least 60%, more preferably at least 65%, 70%,75%, 80%, 85%, 90%, 95%, identity to SEQ ID NO: 3.

The framework is compatible with virtually any rabbit CDRs. Containingdifferent rabbit CDRs, it is well expressed and good produced contraryto the rabbit wild type single chains and still almost fully retains theaffinity of the original donor rabbit antibodies.

The immunobinder acceptor frameworks as described herein may comprisesolubility enhancing substitution in the heavy chain framework,preferably at positions 12, 103 and 144 (AHo numbering). Preferably, ahydrophobic amino acid is substituted by a more hydrophilic amino acid.Hydrophilic amino acids are e.g. Arginine (R), Asparagine (N), Asparticacid (D), Glutamine (Q), Glycine (G), Histidine (H), Lysine (K), Serine(S) and Threonine (T). More preferably, the heavy chain frameworkcomprises (a) Serine (S) at position 12; (b) Serine (S) or Threonine (T)at position 103 and/or (c) Serine (S) or Threonine (T) at position 144.

Moreover, stability enhancing amino acids may be present at one or morepositions 1, 3, 4, 10, 47, 57, 91 and 103 of the variable light chainframework (AHo numbering). More preferably, the variable light chainframework comprises glutamic acid (E) at position 1, valine (V) atposition 3, leucine (L) at position 4, Serine (S) at position 10;Arginine (R) at position 47, Serine (S) at position 57, phenylalanine(F) at position 91 and/or Valine (V) at position 103.

As glutamine (Q) is prone to desamination, in another preferredembodiment, the VH comprises at position 141 a glycine (G). Thissubstitution may improve long-term storage of the protein.

For example, the acceptor frameworks disclosed herein can be used togenerate a human or humanized antibody which retains the bindingproperties of the non-human antibody from which the non-human CDRs arederived. Accordingly, in a preferred embodiment the inventionencompasses an immunobinder acceptor framework as disclosed herein,further comprising heavy chain CDR1, CDR2 and CDR3 and/or light chainCDR1, CDR2 and CDR3 from a donor immunobinder, preferably from amammalian immunobinder, more preferably from a lagomorph immunobinderand most preferably from a rabbit. Thus, in one embodiment, theinvention provides an immunobinder specific to a desired antigencomprising

(i) variable light chain CDRs of a lagomorph; and

(ii) a human variable heavy chain framework having at least 70%,preferably at least 75%, 80%, 85%, 90%, 95%, and most preferably 100%identity to SEQ ID NO: 1.

Preferably, the lagomorph is a rabbit. More preferably, the immunobindercomprises heavy chain CDR1, CDR2 and CDR3 and light chain CDR1, CDR2 andCDR3 from the donor immunobinder.

As known in the art, many rabbit VH chains have extra paired cysteinesrelative to the murine and human counterparts. In addition to theconserved disulfide bridge formed between cys22 and cys92, there is alsoa cys21-cys79 bridge as well as an interCDR S-S bridge formed betweenthe last residue of CDRH1 and the first residue of CDR H2 in some rabbitchains. Besides, pairs of cysteine residues in the CDR-L3 are oftenfound. Besides, many rabbit antibody CDRs do not belong to anypreviously known canonical structure. In particular the CDR-L3 is oftenmuch longer than the CDR-L3 of a human or murine counterpart.

As stated before, the grafting of the non-human CDRs onto the frameworksdisclosed herein yields a molecule wherein the CDRs are displayed in aproper conformation. If required, the affinity of the immunobinder maybe improved by grafting antigen interacting framework residues of thenon-human donor immunobinder. These positions may e.g. be identified by

(i) identifying the respective germ line progenitor sequence or,alternatively, by using the consensus sequences in case of highlyhomologous framework sequences;

(ii) generating a sequence alignment of donor variable domain sequenceswith germ line progenitor sequence or consensus sequence of step (i);and

(iii) identifying differing residues.

Differing residues on the surface of the molecule were in many casesmutated during the affinity generation process in vivo, presumably togenerate affinity to the antigen.

In another aspect, the present invention provides an immunobinder whichcomprises the immunobinder acceptor framework described herein. Saidimmunobinder may e.g. be a scFv antibody, a full-length immunoglobulin,a Fab fragment, a Dab or a Nanobody.

In a preferred embodiment, the immunobinder is attached to one or moremolecules, for example a therapeutic agent such as a cytotoxic agent, acytokine, a chemokine, a growth factor or other signaling molecule, animaging agent or a second protein such as a transcriptional activator ora DNA-binding domain.

The immunobinder as disclosed herein may e.g. be used in diagnosticapplications, therapeutic application, target validation or genetherapy.

The invention further provides an isolated nucleic acid encoding theimmunobinder acceptor framework disclosed herein or the immunobinder(s)as disclosed herein.

In another embodiment, a vector is provided which comprises the nucleicacid disclosed herein.

The nucleic acid or the vector as disclosed herein can e.g. be used ingene therapy.

The invention further encompasses a host cell comprising the vectorand/or the nucleic acid disclosed herein.

Moreover, a composition is provided, comprising the immunobinderacceptor framework as disclosed herein, the immunobinder as disclosedherein, the isolated nucleic acid as disclosed herein or the vector asdisclosed herein.

The sequences disclosed herein are the following (X residues are CDRinsertion sites and contain at least 3 and up to 50 amino acids):

SEQ ID NO. 1: variable heavy chain framework a72EVQLVESGPGLVKPSQTLSLTCGVS(X)_(n=3−50) WIRQHPVKGLEWIG(X)_(n=3−50)RLTISVDTSKTQVSLNLRSVTAADTAVYYCAR(X)_(n=3−50)  WGQGTTVTVSSSEQ ID NO. 2: variable light chain framework K127EIVMTQSPSTLSASVGDRVIITC(X)_(n=3−50)  WYQQKPGKAPKLLIY(X)_(n=3−50)GVPSRFSGSGSGAEFTLTISSLQPDDFATYYC(X)_(n=3−50) FGQGTKLTVLGSEQ ID NO. 3: framework sequence EIVMTQSPSTLSASVGDRVIITC(X)_(n=3−50) WYQQKPGKAPKLLIY(X)_(n=3−50) GVPSRFSGSGSGAEFTLTISSLQPDDFATYYC(X)_(n=3−50)FGQGTKLTVLGGGGGSGGGGSGGGGSGGGGSEVQLVESGPGLVKPSQTLSLTCGVS(X)_(n=3−50)WIRQHPVKGLEWIG(X)_(n=3−50)RLTISVDTSKTQVSLNLRSVTAADTAVYYCAR(X)_(n=3−50)  WGQGTTVTVSSSEQ ID NO: 4: linker GGGGSGGGGSGGGGSGGGGS

In another aspect, the invention provides methods for the humanizationof non-human antibodies by grafting CDRs of non-human donor antibodiesonto stable and soluble antibody frameworks. In a particularly preferredembodiment, the CDRs stem from rabbit antibodies and the frameworks arethose described above.

A general method for grafting CDRs into human acceptor frameworks hasbeen disclosed by Winter in U.S. Pat. No. 5,225,539 and by Queen et al.in WO9007861A1, which are hereby incorporated by reference in theirentirety. The general strategy for grafting CDRs from rabbit monoclonalantibodies onto selected frameworks is related to that of Winter et al.and Queen et al., but diverges in certain key respects. In particular,the methods of the invention diverge from the typical Winter and Queenmethodology known in the art in that the human antibody frameworks asdisclosed herein are particularly suitable as acceptors for human ornon-human donor antibodies. Thus, unlike the general method of Winterand Queen, the framework sequence used for the humanization methods ofthe invention is not necessarily the framework sequence which exhibitsthe greatest sequence similarity to the sequence of the non-human (e.g.,rabbit) antibody from which the donor CDRs are derived. In addition,framework residue grafting from the donor sequence to support CDRconformation is not required. At most, antigen binding amino acidslocated in the framework or other mutations that occurred during somatichypermutation may be introduced.

Particular details of the grafting methods to generate humanizedrabbit-derived antibodies with high solubility and stability aredescribed below.

In exemplary embodiments of the methods of the invention, the amino acidsequence of the CDR donor antibody is first identified and the sequencesaligned using conventional sequence alignment tools (e.g.,Needleman-Wunsch algorithm and Blossum matrices). The introduction ofgaps and nomenclature of residue positions may be done using aconventional antibody numbering system. For example, the AHo numberingsystem for immunoglobulin variable domains may be used. The Kabatnumbering scheme may also be applied since it is the most widely adoptedstandard for numbering the residues in an antibody. Kabat numbering maye.g. be assigned using the SUBIM program. This program analyses variableregions of an antibody sequence and numbers the sequence according tothe system established by Kabat and co-workers (Deret et al 1995). Thedefinition of framework and CDR regions is generally done following theKabat definition which is based on sequence variability and is the mostcommonly used. However, for CDR-H1, the designation is preferably acombination of the definitions of Kabat's, mean contact data generatedby analysis of contacts between antibody and antigen of a subset of 3Dcomplex structures (MacCallum et al., 1996) and Chotia's which is basedon the location of the structural loop regions (see also FIG. 1).Conversion tables for the two different numbering systems used toidentify amino acid residue positions in antibody heavy and light chainvariable regions are provided in A. Honegger, J. Mol. Biol. 309 (2001)657-670. The Kabat numbering system is described further in Kabat et al.(Kabat, E. A., et al. (1991) Sequences of Proteins of ImmunologicalInterest, Fifth Edition, U.S. Department of Health and Human Services,NIH Publication No. 91-3242). The AHo numbering system is describedfurther in Honegger, A. and Pluckthun, A. (2001) J. Mol. Biol.309:657-670).

The variable domains of the rabbit monoclonal antibodies may e.g. beclassified into corresponding human sub-groups using e.g. an EXCELimplementation of sequence analysis algorithms and classificationmethods based on analysis of the human antibody repertoire (Knappik etal., 2000, J Mol Biol. February 11; 296(1):57-86).

CDR conformations may be assigned to the donor antigen binding regions,subsequently residue positions required to maintain the differentcanonical structures can also be identified. The CDR canonicalstructures for five of the six antibody hypervariable regions of rabbitantibodies (L1, L2, L3, H1 and H2) are determined using Chothia's (1989)definition.

The antibodies of the invention may be further optimized to showenhanced functional properties, e.g., enhanced solubility and/orstability. In certain embodiments, the antibodies of the invention areoptimized according to the “functional consensus” methodology disclosedin PCT Application Serial No. PCT/EP2008/001958, entitled “SequenceBased Engineering and Optimization of Single Chain Antibodies”, filed onMar. 12, 2008, which is incorporated herein by reference.

Exemplary framework residue positions for substitution and exemplaryframework substitutions are described in PCT Application No.PCT/CH2008/000285, entitled “Methods of Modifying Antibodies, andModified Antibodies with Improved Functional Properties”, filed on Jun.25, 2008, and PCT Application No. PCT/CH2008/000284, entitled “SequenceBased Engineering and Optimization of Single Chain Antibodies”, filed onJun. 25, 2008.

In other embodiments, the immunobinders of the invention comprise one ormore of the stability enhancing mutations described in U.S. ProvisionalApplication Ser. No. 61/075,692, entitled “Solubility Optimization ofImmunobinders”, filed on Jun. 25, 2008. In certain preferredembodiments, the immunobinder comprises a solubility enhancing mutationat an amino acid position selected from the group of heavy chain aminoacid positions consisting of 12, 103 and 144 (AHo Numbering convention).In one preferred embodiment, the immunobinder comprises one or moresubstitutions selected from the group consisting of: (a) Serine (S) atheavy chain amino acid position 12; (b) Serine (S) or Threonine (T) atheavy chain amino acid position 103; and (c) Serine (S) or Threonine (T)at heavy chain amino acid position 144. In another embodiment, theimmunobinder comprises the following substitutions: (a) Serine (S) atheavy chain amino acid position 12; (b) Serine (S) or Threonine (T) atheavy chain amino acid position 103; and (c) Serine (S) or Threonine (T)at heavy chain amino acid position 144.

In certain preferred embodiments, the immunobinder comprises stabilityenhancing mutations at a framework residue of the light chain acceptorframework in at least one of positions 1, 3, 4, 10, 47, 57, 91 and 103of the light chain variable region according to the AHo numberingsystem. In a preferred embodiment, the light chain acceptor frameworkcomprises one or more substitutions selected from the group consistingof (a) glutamic acid (E) at position 1, (b) valine (V) at position 3,(c) leucine (L) at position 4; (d) Serine (S) at position 10; (e)Arginine (R) at position 47; (e) Serine (S) at position 57; (f)phenylalanine (F) at position 91; and (g) Valine (V) at position 103.

One can use any of a variety of available methods to produce a humanizedantibody comprising a mutation as described above.

Accordingly, the present invention provides an immunobinder humanizedaccording to the method described herein.

In certain preferred embodiments, the target antigen of saidimmunobinder is VEGF or TNFα.

The polypeptides described in the present invention or generated by amethod of the present invention can, for example, be synthesized usingtechniques known in the art. Alternatively nucleic acid moleculesencoding the desired variable regions can be synthesized and thepolypeptides produced by recombinant methods.

For example, once the sequence of a humanized variable region has beendecided upon, that variable region or a polypeptide comprising it can bemade by techniques well known in the art of molecular biology. Morespecifically, recombinant DNA techniques can be used to produce a widerange of polypeptides by transforming a host cell with a nucleic acidsequence (e.g., a DNA sequence that encodes the desired variable region(e.g., a modified heavy or light chain; the variable domains thereof, orother antigen-binding fragments thereof)).

In one embodiment, one can prepare an expression vector including apromoter that is operably linked to a DNA sequence that encodes at leastV_(H) or V_(L). If necessary, or desired, one can prepare a secondexpression vector including a promoter that is operably linked to a DNAsequence that encodes the complementary variable domain (i.e., where theparent expression vector encodes V_(H), the second expression vectorencodes V_(L) and vice versa). A cell line (e.g., an immortalizedmammalian cell line) can then be transformed with one or both of theexpression vectors and cultured under conditions that permit expressionof the chimeric variable domain or chimeric antibody (see, e.g.,International Patent Application No. PCT/GB85/00392 to Neuberger et.al.).

In one embodiment, variable regions comprising donor CDRs and acceptorFR amino acid sequences can be made and then changes introduced into thenucleic acid molecules to effect the CDR amino acid substitution.

Exemplary art recognized methods for making a nucleic acid moleculeencoding an amino acid sequence variant of a polypeptide include, butare not limited to, preparation by site-directed (oroligonucleotide-mediated) mutagenesis, PCR mutagenesis, and cassettemutagenesis of an earlier prepared DNA encoding the polypeptide.

Site-directed mutagenesis is a preferred method for preparingsubstitution variants. This technique is well known in the art (see,e.g., Carter et al. Nucleic Acids Res. 13:4431-4443 (1985) and Kunkel etal., Proc. Natl. Acad. Sci. USA 82:488 (1987)). Briefly, in carrying outsite-directed mutagenesis of DNA, the parent DNA is altered by firsthybridizing an oligonucleotide encoding the desired mutation to a singlestrand of such parent DNA. After hybridization, a DNA polymerase is usedto synthesize an entire second strand, using the hybridizedoligonucleotide as a primer, and using the single strand of the parentDNA as a template. Thus, the oligonucleotide encoding the desiredmutation is incorporated in the resulting double-stranded DNA.

PCR mutagenesis is also suitable for making amino acid sequence variantsof polypeptides. See Higuchi, in PCR Protocols, pp.177-183 (AcademicPress, 1990); and Vallette et al., Nuc. Acids Res. 17:723-733 (1989).Briefly, when small amounts of template DNA are used as startingmaterial in a PCR, primers that differ slightly in sequence from thecorresponding region in a template DNA can be used to generaterelatively large quantities of a specific DNA fragment that differs fromthe template sequence only at the positions where the primers differfrom the template.

Another method for preparing variants, cassette mutagenesis, is based onthe technique described by Wells et al., Gene 34:315-323 (1985). Thestarting material is the plasmid (or other vector) comprising the DNA tobe mutated. The codon(s) in the parent DNA to be mutated are identified.There must be a unique restriction endonuclease site on each side of theidentified mutation site(s). If no such restriction sites exist, theymay be generated using the above-described oligonucleotide-mediatedmutagenesis method to introduce them at appropriate locations in the DNAencoding the polypeptide. The plasmid DNA is cut at these sites tolinearize it. A double-stranded oligonucleotide encoding the sequence ofthe DNA between the restriction sites but containing the desiredmutation(s) is synthesized using standard procedures, wherein the twostrands of the oligonucleotide are synthesized separately and thenhybridized together using standard techniques. This double-strandedoligonucleotide is referred to as the cassette. This cassette isdesigned to have 5′ and 3′ ends that are compatible with the ends of thelinearized plasmid, such that it can be directly ligated to the plasmid.This plasmid now contains the mutated DNA sequence.

A variable region generated by the methods of the invention can bere-modeled and further altered to further increase antigen binding.Thus, the steps described above can be preceded or followed byadditional steps, including, e.g. affinity maturation. In addition,empirical binding data can be used for further optimization.

Aside from amino acid substitutions, the present invention contemplatesother modifications, e.g., to Fc region amino acid sequences in order togenerate an Fc region variant with altered effector function. One may,for example, delete one or more amino acid residues of the Fc region inorder to reduce or enhance binding to an FcR. In one embodiment, one ormore of the Fc region residues can be modified in order to generate suchan Fc region variant. Generally, no more than one to about ten Fc regionresidues will be deleted according to this embodiment of the invention.The Fc region herein comprising one or more amino acid deletions willpreferably retain at least about 80%, and preferably at least about 90%,and most preferably at least about 95%, of the starting Fc region or ofa native sequence human Fc region.

In one embodiment, the polypeptides described in the present inventionor generated by a method of the present invention, e.g., humanized Igvariable regions and/or polypeptides comprising humanized Ig variableregions may be produced by recombinant methods. For example, apolynucleotide sequence encoding a polypeptide can be inserted in asuitable expression vector for recombinant expression. Where thepolypeptide is an antibody, polynucleotides encoding additional lightand heavy chain variable regions, optionally linked to constant regions,may be inserted into the same or different expression vector. Anaffinity tag sequence (e.g. a His(6) tag) may optionally be attached orincluded within the polypeptide sequence to facilitate downstreampurification. The DNA segments encoding immunoglobulin chains are theoperably linked to control sequences in the expression vector(s) thatensure the expression of immunoglobulin polypeptides. Expression controlsequences include, but are not limited to, promoters (e.g.,naturally-associated or heterologous promoters), signal sequences,enhancer elements, and transcription termination sequences. Preferably,the expression control sequences are eukaryotic promoter systems invectors capable of transforming or transfecting eukaryotic host cells.Once the vector has been incorporated into the appropriate host, thehost is maintained under conditions suitable for high level expressionof the nucleotide sequences, and the collection and purification of thepolypeptide.

These expression vectors are typically replicable in the host organismseither as episomes or as an integral part of the host chromosomal DNA.Commonly, expression vectors contain selection markers (e.g.,ampicillin-resistance, hygromycin-resistance, tetracycline resistance orneomycin resistance) to permit detection of those cells transformed withthe desired DNA sequences (see, e.g., U.S. Pat. No. 4,704,362).

E. coli is one prokaryotic host particularly useful for cloning thepolynucleotides (e.g., DNA sequences) of the present invention. Othermicrobial hosts suitable for use include bacilli, such as Bacillussubtilus, and other enterobacteriaceae, such as Salmonella, Serratia,and various Pseudomonas species.

Other microbes, such as yeast, are also useful for expression.Saccharomyces and Pichia are exemplary yeast hosts, with suitablevectors having expression control sequences (e.g., promoters), an originof replication, termination sequences and the like as desired. Typicalpromoters include 3-phosphoglycerate kinase and other glycolyticenzymes. Inducible yeast promoters include, among others, promoters fromalcohol dehydrogenase, isocytochrome C, and enzymes responsible formethanol, maltose, and galactose utilization.

Within the scope of the present invention, E. coli and S. cerevisiae arepreferred host cells.

In addition to microorganisms, mammalian tissue culture may also be usedto express and produce the polypeptides of the present invention (e.g.,polynucleotides encoding immunoglobulins or fragments thereof). SeeWinnacker, From Genes to Clones, VCH Publishers, N.Y., N.Y. (1987).Eukaryotic cells are actually preferred, because a number of suitablehost cell lines capable of secreting heterologous proteins (e.g., intactimmunoglobulins) have been developed in the art, and include CHO celllines, various Cos cell lines, HeLa cells, 293 cells, myeloma celllines, transformed B-cells, and hybridomas. Expression vectors for thesecells can include expression control sequences, such as an origin ofreplication, a promoter, and an enhancer (Queen et al., Immunol. Rev.89:49 (1986)), and necessary processing information sites, such asribosome binding sites, RNA splice sites, polyadenylation sites, andtranscriptional terminator sequences. Preferred expression controlsequences are promoters derived from immunoglobulin genes, SV40,adenovirus, bovine papilloma virus, cytomegalovirus and the like. See Coet al., J. Immunol. 148:1149 (1992).

The vectors containing the polynucleotide sequences of interest (e.g.,the heavy and light chain encoding sequences and expression controlsequences) can be transferred into the host cell by well-known methods,which vary depending on the type of cellular host. For example, calciumchloride transfection is commonly utilized for prokaryotic cells,whereas calcium phosphate treatment, electroporation, lipofection,biolistics or viral-based transfection may be used for other cellularhosts. (See generally Sambrook et al., Molecular Cloning: A LaboratoryManual (Cold Spring Harbor Press, 2nd ed., 1989). Other methods used totransform mammalian cells include the use of polybrene, protoplastfusion, liposomes, electroporation, and microinjection (see generally,Sambrook et al., supra). For production of transgenic animals,transgenes can be microinjected into fertilized oocytes, or can beincorporated into the genome of embryonic stem cells, and the nuclei ofsuch cells transferred into enucleated oocytes.

The subject polypeptide can also be incorporated in transgenes forintroduction into the genome of a transgenic animal and subsequentexpression, e.g., in the milk of a transgenic animal (see, e.g., Deboeret al. U.S. Pat. No. 5,741,957; Rosen U.S. Pat. No. 5,304,489; and MeadeU.S. Pat. No. 5,849,992. Suitable transgenes include coding sequencesfor light and/or heavy chains in operable linkage with a promoter andenhancer from a mammary gland specific gene, such as casein or betalactoglobulin.

Polypeptides can be expressed using a single vector or two vectors. Forexample, antibody heavy and light chains may be cloned on separateexpression vectors and co-transfected into cells.

In one embodiment, signal sequences may be used to facilitate expressionof polypeptides of the invention.

Once expressed, the polypeptides can be purified according to standardprocedures of the art, including ammonium sulfate precipitation,affinity columns (e.g., protein A or protein G), column chromatography,HPLC purification, gel electrophoresis and the like (see generallyScopes, Protein Purification (Springer-Verlag, N.Y., (1982)).

Either the humanized Ig variable regions or polypeptides comprising themcan be expressed by host cells or cell lines in culture. They can alsobe expressed in cells in vivo. The cell line that is transformed (e.g.,transfected) to produce the altered antibody can be an immortalizedmammalian cell line, such as those of lymphoid origin (e.g., a myeloma,hybridoma, trioma or quadroma cell line). The cell line can also includenormal lymphoid cells, such as B-cells, that have been immortalized bytransformation with a virus (e.g., the Epstein-Barr virus).

Although typically the cell line used to produce the polypeptide is amammalian cell line, cell lines from other sources (such as bacteria andyeast) can also be used. In particular, E. coli-derived bacterialstrains can be used, especially, e.g., phage display.

Some immortalized lymphoid cell lines, such as myeloma cell lines, intheir normal state, secrete isolated Ig light or heavy chains. If such acell line is transformed with a vector that expresses an alteredantibody, prepared during the process of the invention, it will not benecessary to carry out the remaining steps of the process, provided thatthe normally secreted chain is complementary to the variable domain ofthe Ig chain encoded by the vector prepared earlier.

If the immortalized cell line does not secrete or does not secrete acomplementary chain, it will be necessary to introduce into the cells avector that encodes the appropriate complementary chain or fragmentthereof.

In the case where the immortalized cell line secretes a complementarylight or heavy chain, the transformed cell line may be produced forexample by transforming a suitable bacterial cell with the vector andthen fusing the bacterial cell with the immortalized cell line (e.g., byspheroplast fusion). Alternatively, the DNA may be directly introducedinto the immortalized cell line by electroporation.

In one embodiment, a humanized Ig variable region as described in thepresent invention or generated by a method of the present invention canbe present in an antigen-binding fragment of any antibody. The fragmentscan be recombinantly produced and engineered, synthesized, or producedby digesting an antibody with a proteolytic enzyme. For example, thefragment can be a Fab fragment; digestion with papain breaks theantibody at the region, before the inter-chain (i.e., V_(H)-V_(H))disulphide bond, that joins the two heavy chains. This results in theformation of two identical fragments that contain the light chain andthe V_(H) and C_(H)1 domains of the heavy chain. Alternatively, thefragment can be an F(ab′)₂ fragment. These fragments can be created bydigesting an antibody with pepsin, which cleaves the heavy chain afterthe inter-chain disulfide bond, and results in a fragment that containsboth antigen-binding sites. Yet another alternative is to use a “singlechain” antibody. Single-chain Fv (scFv) fragments can be constructed ina variety of ways. For example, the C-terminus of V_(H) can be linked tothe N-terminus of V_(L). Typically, a linker (e.g., (GGGGS)₄; SEQ ID NO:4) is placed between V_(H) and V_(L). However, the order in which thechains can be linked can be reversed, and tags that facilitate detectionor purification (e.g., Myc-, His-, or FLAG-tags) can be included (tagssuch as these can be appended to any antibody or antibody fragment ofthe invention; their use is not restricted to scFv). Accordingly, and asnoted below, tagged antibodies are within the scope of the presentinvention. In alternative embodiments, the antibodies described herein,or generated by the methods described herein, can be heavy chain dimersor light chain dimers. Still further, an antibody light or heavy chain,or portions thereof, for example, a single domain antibody (DAb), can beused.

In another embodiment, a humanized Ig variable region as described inthe present invention or generated by a method of the present inventionis present in a single chain antibody (ScFv) or a minibody (see e.g.,U.S. Pat. No. 5,837,821 or WO 94/09817A1). Minibodies are dimericmolecules made up of two polypeptide chains each comprising an ScFvmolecule (a single polypeptide comprising one or more antigen bindingsites, e.g., a V_(L) domain linked by a flexible linker to a V_(H)domain fused to a CH3 domain via a connecting peptide). ScFv moleculescan be constructed in a V_(H)-linker-V_(L) orientation orV_(L)-linker-V_(H) orientation. The flexible hinge that links the V_(L)and V_(H) domains that make up the antigen binding site preferablycomprises from about 10 to about 50 amino acid residues. An exemplaryconnecting peptide for this purpose is (Gly4Ser)3 (Huston et al. (1988).PNAS, 85:5879). Other connecting peptides are known in the art.

Methods of making single chain antibodies are well known in the art,e.g., Ho et al. (1989), Gene, 77:51; Bird et al. (1988), Science242:423; Pantoliano et al. (1991), Biochemistry 30:10117; Milenic et al.(1991), Cancer Research, 51:6363; Takkinen et al. (1991), ProteinEngineering 4:837. Minibodies can be made by constructing an ScFvcomponent and connecting peptide-CH₃ component using methods describedin the art (see, e.g., U.S. Pat. No. 5,837,821 or WO 94/09817A1). Thesecomponents can be isolated from separate plasmids as restrictionfragments and then ligated and recloned into an appropriate vector.Appropriate assembly can be verified by restriction digestion and DNAsequence analysis. In one embodiment, a minibody of the inventioncomprises a connecting peptide. In one embodiment, the connectingpeptide comprises a Gly/Ser linker, e.g., GGGSSGGGSGG (SEQ ID NO: 6).

In another embodiment, a tetravalent minibody can be constructed.Tetravalent minibodies can be constructed in the same manner asminibodies, except that two ScFv molecules are linked using a flexiblelinker, e.g., having an amino acid sequence (G₄S)₄G₃AS (SEQ ID NO: 7).

In another embodiment, a humanized variable region as described in thepresent invention or generated by a method of the present invention canbe present in a diabody. Diabodies are similar to scFv molecules, butusually have a short (less than 10 and preferably 1-5) amino acidresidue linker connecting both variable domains, such that the V_(L) andV_(H) domains on the same polypeptide chain can not interact. Instead,the V_(L) and V_(H) domain of one polypeptide chain interact with theV_(H) and V_(L) domain (respectively) on a second polypeptide chain (WO02/02781).

In another embodiment, a humanized variable region of the invention canbe present in an immunoreactive fragment or portion of an antibody (e.g.an scFv molecule, a minibody, a tetravalent minibody, or a diabody)operably linked to an FcR binding portion. In an exemplary embodiment,the FcR binding portion is a complete Fc region.

Preferably, the humanization methods described herein result in Igvariable regions in which the affinity for antigen is not substantiallychanged compared to the donor antibody.

In one embodiment, polypeptides comprising the variable domains of theinstant invention bind to antigens with a binding affinity greater than(or equal to) an association constant Ka of about 10⁵ M⁻¹, 10⁶ M⁻¹, 10⁷M⁻¹, 10⁸ M⁻¹, 10⁹ M⁻¹, 10¹⁰ M⁻¹, 10¹¹ M⁻¹, or 10¹² M⁻¹, (includingaffinities intermediate of these values).

Affinity, avidity, and/or specificity can be measured in a variety ofways. Generally, and regardless of the precise manner in which affinityis defined or measured, the methods of the invention improve antibodyaffinity when they generate an antibody that is superior in any aspectof its clinical application to the antibody (or antibodies) from whichit was made (for example, the methods of the invention are consideredeffective or successful when a modified antibody can be administered ata lower dose or less frequently or by a more convenient route ofadministration than an antibody (or antibodies) from which it was made).

More specifically, the affinity between an antibody and an antigen towhich it binds can be measured by various assays, including, e.g., anELISA assay, a BiaCore assay or the KinExA™ 3000 assay (available fromSapidyne Instruments (Boise, Id.)). Briefly, sepharose beads are coatedwith antigen (the antigen used in the methods of the invention can beany antigen of interest (e.g., a cancer antigen; a cell surface proteinor secreted protein; an antigen of a pathogen (e.g., a bacterial orviral antigen (e.g., an HIV antigen, an influenza antigen, or ahepatitis antigen)), or an allergen) by covalent attachment. Dilutionsof antibody to be tested are prepared and each dilution is added to thedesignated wells on a plate. A detection antibody (e.g. goat anti-humanIgG-HRP conjugate) is then added to each well followed by a chromagenicsubstrate (e.g. HRP). The plate is then read in ELISA plate reader at450 nM, and EC50 values are calculated. (It is understood, however, thatthe methods described here are generally applicable; they are notlimited to the production of antibodies that bind any particular antigenor class of antigens.)

Those of ordinary skill in the art will recognize that determiningaffinity is not always as simple as looking at a single figure. Sinceantibodies have two arms, their apparent affinity is usually much higherthan the intrinsic affinity between the variable region and the antigen(this is believed to be due to avidity). Intrinsic affinity can bemeasured using scFv or Fab fragments.

In another aspect, the present invention features a humanized rabbitantibody, or a fragment thereof, conjugated to a therapeutic moiety,such as a cytotoxin, a drug (e.g., an immunosuppressant) or aradiotoxin. Such conjugates are referred to herein as“immunoconjugates”.

The antibody conjugates of the invention can be used to modify a givenbiological response, and the drug moiety is not to be construed aslimited to classical chemical therapeutic agents. For example, the drugmoiety may be a protein or polypeptide possessing a desired biologicalactivity. Such proteins may include, for example, an enzymaticallyactive toxin, or active fragment thereof, such as abrin, ricin A,pseudomonas exotoxin, or diphtheria toxin; a protein such as tumornecrosis factor or interferon-γ; or, biological response modifiers suchas, for example, lymphokines, interleukin-1 (“IL-1”), interleukin-2(“IL-2”), interleukin-6 (“IL-6”), granulocyte macrophage colonystimulating factor (“GM-CSF”), granulocyte colony stimulating factor(“G-CSF”), or other growth factors.

Techniques for conjugating such therapeutic moiety to antibodies arewell known, see, e.g., Arnon et al., “Monoclonal Antibodies ForImmunotargeting Of Drugs In Cancer Therapy”, in Monoclonal AntibodiesAnd Cancer Therapy, Reisfeld et al. (eds.), pp. 243-56 (Alan R. Liss,Inc. 1985); Hellstrom et al., “Antibodies For Drug Delivery”, inControlled Drug Delivery (2nd Ed.), Robinson et al. (eds.), pp. 623-53(Marcel Dekker, Inc. 1987); Thorpe, “Antibody Carriers Of CytotoxicAgents In Cancer Therapy: A Review”, in Monoclonal Antibodies '84:Biological And Clinical Applications, Pinchera et al. (eds.), pp.475-506 (1985); “Analysis, Results, And Future Prospective Of TheTherapeutic Use Of Radiolabeled Antibody In Cancer Therapy”, inMonoclonal Antibodies For Cancer Detection And Therapy, Baldwin et al.(eds.), pp. 303-16 (Academic Press 1985), and Thorpe et al., “ThePreparation And Cytotoxic Properties Of Antibody-Toxin Conjugates”,Immunol. Rev., 62:119-58 (1982).

In one aspect the invention provides pharmaceutical formulationscomprising humanized rabbit antibodies for the treatment disease. Theterm “pharmaceutical formulation” refers to preparations which are insuch form as to permit the biological activity of the antibody orantibody derivative to be unequivocally effective, and which contain noadditional components which are toxic to the subjects to which theformulation would be administered. “Pharmaceutically acceptable”excipients (vehicles, additives) are those which can reasonably beadministered to a subject mammal to provide an effective dose of theactive ingredient employed.

EQUIVALENTS

Numerous modifications and alternative embodiments of the presentinvention will be apparent to those skilled in the art in view of theforegoing description. Accordingly, this description is to be construedas illustrative only and is for the purpose of teaching those skilled inthe art the best mode for carrying out the present invention. Details ofthe structure may vary substantially without departing from the spiritof the invention, and exclusive use of all modifications that comewithin the scope of the appended claims is reserved. It is intended thatthe present invention be limited only to the extent required by theappended claims and the applicable rules of law.

All literature and similar material cited in this application,including, patents, patent applications, articles, books, treatises,dissertations, web pages, figures and/or appendices, regardless of theformat of such literature and similar materials, are expresslyincorporated by reference in their entirety. In the event that one ormore of the incorporated literature and similar materials differs fromor contradicts this application, including defined terms, term usage,described techniques, or the like, this application controls.

1. A human heavy chain acceptor framework comprising SEQ ID NO:
 1. 2.The human heavy chain acceptor framework of claim 1, comprising an aminoacid substitution at position 12, 103, and/or 144 (Aho numbering). 3.The human heavy chain acceptor framework of claim 2, wherein thesubstitution is (a) Serine (S) at position 12; (b) Serine (S) orThreonine (T) at position 103; and/or (c) Serine (S) or Threonine (T) atposition
 144. 4. An isolated nucleic acid encoding the acceptorframework of claim
 1. 5. A vector comprising the nucleic acid of claim4.
 6. A host cell comprising the vector of claim
 5. 7. An immunobinderspecific to a desired antigen comprising: (a) a light chain acceptorframework comprising variable light chain CDRs of a lagomorphimmunobinder; and (b) human heavy chain acceptor framework of claim 1comprising variable heavy chain CDRs of a lagomorph immunobinder.
 8. Theimmunobinder of claim 7, wherein the light chain acceptor framework hasat least 85% identity to SEQ ID NO:
 2. 9. The immunobinder of claim 7,further comprising a linker sequence that links the variable light chainframework and the heavy chain acceptor framework, wherein the linkersequence is SEQ ID NO:
 4. 10. The immunobinder of claim 7, furthercomprising donor framework residues involved in antigen binding.
 11. Theimmunobinder of claim 7, wherein the immunobinder is a scFv antibody, afull-length immunoglobulin or a Fab fragment.
 12. A method of humanizinga rabbit immunobinder, the method comprising: (a) grafting at least oneheavy chain CDR of the group consisting of CDR H1, CDR H2 and CDR H3sequences from a donor rabbit immunobinder into the human heavy chainacceptor framework of claim 1; and (b) grafting at least one light chainCDR of the group consisting of CDR L1, CDR L2 and CDR L3 sequences froma donor rabbit immunobinder into a human light chain acceptor frameworkinto a light chain acceptor framework has at least 85% identity to SEQID NO:
 2. 13. The method of claim 12, further comprising substitutingframework residues in one or both of the human heavy chain acceptorframework and the human light chain framework with framework residues ofthe donor rabbit immunobinder.
 14. The method of claim 12, wherein theheavy chain acceptor framework has a substitution at one or more ofheavy chain amino positions 12, 103 and 144 (AHo numbering).
 15. Themethod of claim 14, wherein the substitution at one or more of positions12, 103 and 144 are selected from the group consisting of: (a) Serine(S) at position 12; (b) Threonine (T) at position 103; and (c) Threonine(T) at position
 144. 16. An immunobinder humanized according to themethod of claim 12.